CRYOCARE®
Biomimetic Shield Against Thermal Stress
INCI
Glycerin, Aqua, Hippophae rhamnoides fruit extract
Cold & heat defense
barrier protection
high-tech complex
CRYOCARE® is a high-tech active ingredient designed to help the skin withstand extreme climatic conditions — from freezing temperatures to heat and IR exposure. Its climate-adapted phytocomplex, derived from sea-buckthorn and refined through an eco-designed extraction process, supports essential mechanisms involved in the epidermal response to cold shock, heat stress and rapid thermal transitions.
By enhancing the skin’s resilience under these stressors, CRYOCARE® reinforces barrier performance, limits protein and lipid fatigue, and helps preserve Collagen I integrity — promoting a stronger, more stable and visibly healthier skin structure.
EFFICACY TESTS
Cold-Proof Skin Protection — Cell Viability
Setup:
Human keratinocytes exposed to extreme hypothermic stress (–20 °C, 1 h, one or two cycles) with or without CRYOCARE®.
Interpretation:
CRYOCARE® markedly improves survival capacity under freeze stress:
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+176% viability (1 cycle)
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+342% viability (2 cycles)
Thermal Protective Effect — Skin Hydration
Setup:
Baseline assessment, then skin treated with CRYOCARE® (5%) or placebo and exposed to controlled cold and dry conditions (12 ± 2 °C; 35 ± 5% RH). Hydration measured by Corneometer®.
Interpretation:
CRYOCARE® increases moisture even in cold conditions:
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+44.6% hydration vs untreated
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Hydration doubled vs placebo
IR-Induced Stress — HSP72 Regulation
Setup:
Keratinocytes exposed to heat + IR stress (≥42 °C, IR 300 J/m², 60 min).
Interpretation:
CRYOCARE® significantly decreases the stress marker HSP72:
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–57.2% (0.15%)
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–60.0% (0.3%)
Thermal Transition Multi-Endpoint Evaluation
A multi-endpoint model replicating extreme climatic stress (–20 °C → +42 °C) simultaneously assessed barrier integrity, structural cohesion and collagen preservation — providing a comprehensive view of skin performance under rapid temperature shifts.
Barrier Integrity — TEER (3D Tissue)
Setup: TEER measured on 3D reconstructed epidermis exposed to thermal shock.
Interpretation: +39% to +55% TEER protection.
Structural Cohesion — Histology
A 3D full-thickness skin model was exposed to a cold–heat transition (–20 °C → +42 °C). Tissue morphology was assessed through histological staining to evaluate degeneration, erosion and overall structural cohesion. A quantitative bar chart was produced to report the degree of structural damage with and without CRYOCARE®.
Graph Interpretation (Bar Chart)
CRYOCARE® reduces structural deterioration in a clear, dose-dependent pattern, showing markedly lower levels of degeneration compared to untreated tissue.
Image Interpretation (Histology Microscopy)
Microscopy images confirm a more intact epidermal architecture, with fewer lesions, reduced necrosis and better cohesion across layers.
CONTROL
COLD-TO-HEAT STRESSED
COLD-TO-HEAT STRESSED 1.25% CRYOCARE
COLD-TO-HEAT STRESSED 2.5% CRYOCARE
Collagen I Preservation — Fluorescence
Collagen I content was quantified after cold–heat stress using fluorescence imaging, and expressed through a radar chart comparing untreated tissue and CRYOCARE® at increasing concentrations. Microscopy images (fluorescence) visualize collagen fiber preservation.
Graph Interpretation (Radar Chart)
CRYOCARE® protects collagen I from thermal degradation, preserving +52% (1.25%) and +74% (2.5%) compared to untreated tissue subjected to the same shock.
Image Interpretation (Fluorescence Microscopy)
Fluorescence images show a visibly higher density and organization of collagen fibers in CRYOCARE®-treated tissue versus degraded collagen in controls.
CONTROL
COLD-TO-HEAT STRESSED
COLD-TO-HEAT STRESSED 1.25% CRYOCARE
COLD-TO-HEAT STRESSED 2.5% CRYOCARE
Key Product Details
% OF USE
2% – 5%
APPLICATIONS
Ideal for emulsions or transparent water-based formulations
TECHNICAL DATA
| APPEARANCE | CLEAR LIQUID |
| COLOUR | LIGHT AMBER |
| ODOUR | CHARACTERISTIC |
| DRY RESIDUE (105°C) | > 90 % |
| SPECIFIC GRAVITY (25°C) | 1.2 – 1.3 g/ml |
| PH (5% WATER SOLUTION AT 25°C) | 4 – 6 |
PROPERTIES
Thermal Shock Adaptation
Supports the skin’s ability to respond to rapid cold–heat transitions and environmental temperature fluctuations.
Barrier Performance Support
Contributes to maintaining epidermal function under thermal stress, helping the skin remain stable and comfortable.
Structural Stability
Helps limit tissue disruption associated with extreme climatic exposure, promoting a more cohesive skin architecture.
Collagen I Preservation
Assists in minimizing thermally induced collagen breakdown, contributing to long-term firmness.
Hydration & Comfort in Cold Climates
Improves skin feel and moisture levels in low-temperature environments.
High-Tech & Safe
Refined biomimetic active obtained from Hippophae rhamnoides through an eco-designed process; clean profile and easy to integrate into modern formulations.
EN 
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